產(chǎn)品詳情
簡單介紹:
Mouse TARC (Thymus Activation Regulated Chemokine) Sandwich ELISA (STJE0005320)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 37.5pg/mL |
Detection Limit: | 62.5~4000pg/mL |
Short Description: | This TARC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Ccl17 |
Gene ID: | 20295 |
Uniprot ID: | CCL17_MOUSE |
Specificity: | This kit recognizes Mouse TARC in samples. No significant cross-reactivity or interference between Mouse TARC and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes. Acts by binding to CCR4 at T-cell surface. Mediates GM-CSF/CSF2-driven pain and inflammation. In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation. Plays a role in wound healing, mainly by inducing fibroblast migration into the wound. |
Protein Name | C-C Motif Chemokine 17 Abcd-2 Cc Chemokine Tarc Small-Inducible Cytokine A17 Thymus And Activation-Regulated Chemokine |
Cellular Localisation | Secreted |
Alternative ELISA Names | C-C Motif Chemokine 17 ELISA kit Abcd-2 ELISA kit Cc Chemokine Tarc ELISA kit Small-Inducible Cytokine A17 ELISA kit Thymus And Activation-Regulated Chemokine ELISA kit Ccl17 ELISA kit Tarc ELISA kit |
Specificity | This kit recognizes Mouse TARC in samples. No significant cross-reactivity or interference between Mouse TARC and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 90-101 | 98 |
EDTA plasma (n=8) | 98-112 | 105 |
Cell culture media (n=8) | 91-106 | 98 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 184.72 | 464.66 | 1705.04 | 199.08 | 441.91 | 1746.04 |
Standard deviation | 9.90 | 21.65 | 77.41 | 10.79 | 22.01 | 78.57 |
CV (%) | 5.36 | 4.66 | 4.54 | 5.42 | 4.98 | 4.50 |
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