產(chǎn)品詳情
簡單介紹:
Mouse Ctsk (Cathepsin K) Sandwich ELISA (STJE0004376)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This Ctsk Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Ctsk |
Gene ID: | 13038 |
Uniprot ID: | CATK_MOUSE |
Specificity: | This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Thiol protease involved in osteoclastic bone resorption. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. |
Protein Name | Cathepsin K |
Database Links | Reactome: R-MMU-1442490 Reactome: -MMU-1474228 Reactome: -MMU-1592389 Reactome: -MMU-1679131 Reactome: -MMU-2132295 Reactome: -MMU-8939242 |
Cellular Localisation | Lysosome Secreted Apical Cell Membrane Peripheral Membrane Protein Extracellular Side Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
Alternative ELISA Names | Cathepsin K ELISA kit Ctsk ELISA kit |
Specificity | This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 90-102 | 95 |
EDTA plasma (n=8) | 93-103 | 98 |
Cell culture media (n=8) | 85-99 | 92 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 51.87 | 135.85 | 457.69 | 53.87 | 133.39 | 422.39 |
Standard deviation | 3.53 | 5.83 | 24.49 | 3.52 | 5.44 | 18.50 |
CV (%) | 6.81 | 4.29 | 5.35 | 6.53 | 4.08 | 4.38 |
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