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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse vWF (von Willebrand Factor) CLIA Kit (STJC0001486)

  • 產(chǎn)品型號：STJC0001486
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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簡單介紹：
Mouse vWF (von Willebrand Factor) CLIA Kit (STJC0001486)
詳情介紹：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This mouse vWF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Vwf
Gene ID: 22371
Uniprot ID: VWF_MOUSE
Specificity: This kit recognizes Mouse vWF in samples. No significant cross-reactivity or interference between Mouse vWF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Plasma. Expressed in liver.
Post Translational Modifications All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated.
Function Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Protein Name Von Willebrand Factor
Vwf Cleaved Into - Von Willebrand Antigen 2
Von Willebrand Antigen Ii
Database Links Reactome: R-MMU-114608
Reactome: -MMU-140837
Reactome: -MMU-216083
Reactome: -MMU-354192
Reactome: -MMU-354194
Reactome: -MMU-372708
Reactome: -MMU-430116
Reactome: -MMU-5674135
Reactome: -MMU-75892
Reactome: -MMU-76009
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Localized To Storage Granules
Alternative CLIA Names Von Willebrand Factor CLIA kit
Vwf Cleaved Into - Von Willebrand Antigen 2 CLIA kit
Von Willebrand Antigen Ii CLIA kit
Vwf CLIA kit
Specificity This kit recognizes Mouse vWF in samples. No significant cross-reactivity or interference between Mouse vWF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 97
EDTA plasma(n=8) 88-100 94
Cell culture media(n=8) 86-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.96 2.29 9.10 0.94 2.27 9.14
Standard deviation 0.10 0.21 1.00 0.12 0.23 0.64
CV (%) 10.42 9.17 10.99 12.77 10.13 7.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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