產(chǎn)品詳情
簡(jiǎn)單介紹：
Mouse Ucp1 (Mitochondrial brown fat uncoupling protein 1) Sandwich ELISA (STJE0004733)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This Ucp1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Ucp1 |
| Gene ID: | 22227 |
| Uniprot ID: | UCP1_MOUSE |
| Specificity: | This kit recognizes Mouse UCP-1 in samples. No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Sulfenylation at Cys-254 is increased upon cold exposure. It increases the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects. May undergo ubiquitin-mediated proteasomal degradation. |
| Function | Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Regulates the production of reactive oxygen species/ROS by mitochondria. |
| Protein Name | Mitochondrial Brown Fat Uncoupling Protein 1 Ucp 1 Solute Carrier Family 25 Member 7 Thermogenin |
| Database Links | Reactome: R-MMU-167826 Reactome: -MMU-167827 |
| Cellular Localisation | Mitochondrion Inner Membrane Multi-Pass Membrane Protein |
| Alternative ELISA Names | Mitochondrial Brown Fat Uncoupling Protein 1 ELISA kit Ucp 1 ELISA kit Solute Carrier Family 25 Member 7 ELISA kit Thermogenin ELISA kit Ucp1 ELISA kit Slc25a7 ELISA kit Ucp ELISA kit |
| Specificity | This kit recognizes Mouse UCP-1 in samples. No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma (n=8) | 95-106 | 101 |
| Cell culture media (n=8) | 87-98 | 92 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 0.93 | 4.14 | 0.47 | 1.00 | 3.90 |
| Standard deviation | 0.02 | 0.04 | 0.16 | 0.03 | 0.06 | 0.13 |
| CV (%) | 4.17 | 4.30 | 3.86 | 6.38 | 6.00 | 3.33 |
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