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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse TRY (Tyrosinase) CLIA Kit (STJC0001478)

  • 產(chǎn)品型號(hào)：STJC0001478
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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Mouse TRY (Tyrosinase) CLIA Kit (STJC0001478)
詳情介紹：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This mouse TRY kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Tyr
Gene ID: 22173
Uniprot ID: TYRO_MOUSE
Specificity: This kit recognizes Mouse TRY in samples. No significant cross-reactivity or interference between Mouse TRY and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the skin.
Post Translational Modifications Glycosylated.
Function This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Protein Name Tyrosinase
Albino Locus Protein
Monophenol Monooxygenase
Database Links Reactome: R-MMU-5662702
Cellular Localisation Melanosome Membrane
Single-Pass Type I Membrane Protein
Melanosome
Proper Trafficking To Melanosome Is Regulated By Sgsm2
Ankrd27
Rab9a
Rab32 And Rab38
Alternative CLIA Names Tyrosinase CLIA kit
Albino Locus Protein CLIA kit
Monophenol Monooxygenase CLIA kit
Tyr CLIA kit
Specificity This kit recognizes Mouse TRY in samples. No significant cross-reactivity or interference between Mouse TRY and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-100 95
EDTA plasma(n=8) 85-100 91
Cell culture media(n=8) 85-97 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 110.91 273.90 949.56 120.67 297.52 918.02
Standard deviation 11.97 24.73 72.64 13.21 27.64 64.17
CV (%) 10.79 9.03 7.65 10.95 9.29 6.99
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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