產(chǎn)品詳情
簡單介紹：
Mouse Trx (Thioredoxin) CLIA Kit (STJC0001451)
詳情介紹：
| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This mouse Trx kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Txn |
| Gene ID: | 22166 |
| Uniprot ID: | THIO_MOUSE |
| Specificity: | This kit recognizes Mouse Trx in samples. No significant cross-reactivity or interference between Mouse Trx and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). |
| Protein Name | Thioredoxin Trx Atl-Derived Factor Adf |
| Database Links | Reactome: R-MMU-2559580 Reactome: -MMU-3299685 Reactome: -MMU-499943 Reactome: -MMU-5628897 Reactome: -MMU-5676934 Reactome: -MMU-844456 |
| Cellular Localisation | Nucleus Cytoplasm Secreted Translocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma) Predominantly In The Cytoplasm In Non Irradiated Cells Radiation Induces Translocation Of Trx From The Cytoplasm To The Nucleus Secreted By A Leaderless Secretory Pathway |
| Alternative CLIA Names | Thioredoxin CLIA kit Trx CLIA kit Atl-Derived Factor CLIA kit Adf CLIA kit Txn CLIA kit Txn1 CLIA kit |
| Specificity | This kit recognizes Mouse Trx in samples. No significant cross-reactivity or interference between Mouse Trx and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 95-110 | 101 |
| EDTA plasma(n=8) | 90-101 | 95 |
| Cell culture media(n=8) | 84-96 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 52.17 | 93.85 | 448.69 | 53.64 | 91.71 | 417.16 |
| Standard deviation | 6.00 | 10.28 | 48.01 | 6.70 | 8.19 | 36.92 |
| CV (%) | 11.50 | 10.95 | 10.70 | 12.49 | 8.93 | 8.85 |
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