產(chǎn)品詳情
簡單介紹：
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) Sandwich ELISA (STJE0019692)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13-5000pg/mL |
| Short Description: | This TIMP-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Timp1 |
| Gene ID: | 21857 |
| Uniprot ID: | TIMP1_MOUSE |
| Specificity: | This kit recognizes Mouse TIMP-1 in samples. No significant cross-reactivity or interference between Mouse TIMP-1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. |
| Function | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. |
| Protein Name | Metalloproteinase Inhibitor 1 Collagenase Inhibitor 16c8 Fibroblast Erythroid-Potentiating Activity Epa Tpa-S1 Tpa-Induced Protein Tissue Inhibitor Of Metalloproteinases 1 Timp-1 |
| Database Links | Reactome: R-MMU-114608 Reactome: -MMU-1592389 Reactome: -MMU-381426 Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Metalloproteinase Inhibitor 1 ELISA kit Collagenase Inhibitor 16c8 Fibroblast ELISA kit Erythroid-Potentiating Activity ELISA kit Epa ELISA kit Tpa-S1 ELISA kit Tpa-Induced Protein ELISA kit Tissue Inhibitor Of Metalloproteinases 1 ELISA kit Timp-1 ELISA kit Timp1 ELISA kit Timp ELISA kit Timp-1 ELISA kit |
| Specificity | This kit recognizes Mouse TIMP-1 in samples. No significant cross-reactivity or interference between Mouse TIMP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 85-100 | 92 |
| EDTA plasma (n=8) | 88-100 | 94 |
| Cell culture media (n=8) | 91-106 | 97 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 248.17 | 730.69 | 2077.72 | 233.39 | 783.18 | 2236.48 |
| Standard deviation | 12.56 | 43.33 | 100.35 | 15.47 | 41.67 | 108.47 |
| CV (%) | 5.06 | 5.93 | 4.83 | 6.63 | 5.32 | 4.85 |
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