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產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：Mouse Tgfa (Protransforming growth factor alpha) Sandwich ELISA (STJE0005070)

  • 產(chǎn)品型號(hào)：STJE0005070
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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Mouse Tgfa (Protransforming growth factor alpha) Sandwich ELISA (STJE0005070)
詳情介紹：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Tgfa Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Tgfa
Gene ID: 21802
Uniprot ID: TGFA_MOUSE
Specificity: This kit recognizes Mouse TGF-Alpha in samples. No significant cross-reactivity or interference between Mouse TGF-Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Protransforming Growth Factor Alpha Cleaved Into - Transforming Growth Factor Alpha
Tgf-Alpha
Egf-Like Tgf
Etgf
Tgf Type 1
Database Links Reactome: R-MMU-1257604
Reactome: -MMU-177929
Reactome: -MMU-179812
Reactome: -MMU-180292
Reactome: -MMU-180336
Reactome: -MMU-182971
Reactome: -MMU-204005
Reactome: -MMU-212718
Reactome: -MMU-5673001
Reactome: -MMU-5694530
Reactome: -MMU-6811558
Reactome: -MMU-8856825
Reactome: -MMU-8856828
Reactome: -MMU-9009391
Cellular Localisation Transforming Growth Factor Alpha: Secreted
Extracellular Space
Protransforming Growth Factor Alpha: Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Protransforming Growth Factor Alpha Cleaved Into - Transforming Growth Factor Alpha ELISA kit
Tgf-Alpha ELISA kit
Egf-Like Tgf ELISA kit
Etgf ELISA kit
Tgf Type 1 ELISA kit
Tgfa ELISA kit
Specificity This kit recognizes Mouse TGF-α in samples. No significant cross-reactivity or interference between Mouse TGF-α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-98 90
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 87-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.46 96.38 484.14 57.00 100.72 506.31
Standard deviation 3.79 4.39 20.04 3.88 4.57 25.06
CV (%) 6.96 4.55 4.14 6.81 4.54 4.95
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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