產(chǎn)品詳情
簡單介紹：
Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This Sst Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
| Gene Symbol: | Sst |
| Gene ID: | 20604 |
| Uniprot ID: | SMS_MOUSE |
| Specificity: | This kit recognizes Mouse SST in samples. No significant cross-reactivity or interference between Mouse SST and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | C-terminal amidation of the neuronostatin peptide is required for its biological activity, including for the regulation of mean arterial pressure. |
| Function | Somatostatin-14: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH.the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin: May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH. |
| Protein Name | Somatostatin Cleaved Into - Antrin - Somatostatin-28 - Somatostatin-14 - Neuronostatin Nst |
| Database Links | Reactome: R-MMU-375276 Reactome: -MMU-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Somatostatin Cleaved Into - Antrin - Somatostatin-28 - Somatostatin-14 - Neuronostatin ELISA kit Nst ELISA kit Sst ELISA kit Smst ELISA kit |
| Specificity | This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma(n=8) | 84-97 | 90 |
| Cell culture media(n=8) | 86-96 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 26.50 | 74.00 | 196.80 | 28.70 | 68.50 | 190.70 |
| Standard deviation | 1.40 | 4.10 | 10.20 | 1.50 | 2.70 | 8.40 |
| CV (%) | 5.28 | 5.54 | 5.18 | 5.23 | 3.94 | 4.40 |
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