產(chǎn)品詳情
簡(jiǎn)單介紹:
Mouse Snca (Alpha-synuclein) Sandwich ELISA (STJE0005009)
詳情介紹:
Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This Snca Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Snca |
Gene ID: | 20617 |
Uniprot ID: | SYUA_MOUSE |
Specificity: | This kit recognizes Mouse SNC Alpha in samples. No significant cross-reactivity or interference between Mouse SNC Alpha and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
Protein Name | Alpha-Synuclein Non-A Beta Component Of Ad Amyloid Non-A4 Component Of Amyloid Precursor Nacp |
Cellular Localisation | Cytoplasm Membrane Nucleus Synapse Secreted Cell Projection Axon Membrane-Bound In Dopaminergic Neurons Expressed And Colocalized With Septin4 In Dopaminergic Axon Terminals Especially At The Varicosities |
Alternative ELISA Names | Alpha-Synuclein ELISA kit Non-A Beta Component Of Ad Amyloid ELISA kit Non-A4 Component Of Amyloid Precursor ELISA kit Nacp ELISA kit Snca ELISA kit Syn ELISA kit |
Specificity | This kit recognizes Mouse SNCα in samples. No significant cross-reactivity or interference between Mouse SNCα and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 88-104 | 95 |
EDTA plasma (n=8) | 89-105 | 96 |
Cell culture media (n=8) | 83-97 | 90 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 49.82 | 94.36 | 446.10 | 46.36 | 92.27 | 467.95 |
Standard deviation | 2.65 | 3.82 | 16.19 | 2.86 | 4.48 | 23.54 |
CV (%) | 5.32 | 4.05 | 3.63 | 6.17 | 4.86 | 5.03 |
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