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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse Sftpd (Pulmonary surfactant-associated protein D) Sandwich ELISA (STJE0004933)

  • 產(chǎn)品型號：STJE0004933
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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簡單介紹：
Mouse Sftpd (Pulmonary surfactant-associated protein D) Sandwich ELISA (STJE0004933)
詳情介紹：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Sftpd Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Sftpd
Gene ID: 20390
Uniprot ID: SFTPD_MOUSE
Specificity: This kit recognizes Mouse SP-D in samples. No significant cross-reactivity or interference between Mouse SP-D and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Protein Name Pulmonary Surfactant-Associated Protein D
Psp-D
Sp-D
Lung Surfactant Protein D
Database Links Reactome: R-MMU-166016
Reactome: -MMU-198933
Reactome: -MMU-391160
Reactome: -MMU-5683826
Reactome: -MMU-5686938
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Surface Film
Alternative ELISA Names Pulmonary Surfactant-Associated Protein D ELISA kit
Psp-D ELISA kit
Sp-D ELISA kit
Lung Surfactant Protein D ELISA kit
Sftpd ELISA kit
Sftp4 ELISA kit
Specificity This kit recognizes Mouse SP-D in samples. No significant cross-reactivity or interference between Mouse SP-D and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma (n=8) 86-100 92
Cell culture media (n=8) 91-105 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.52 1.22 3.35 0.50 1.14 3.53
Standard deviation 0.03 0.06 0.11 0.03 0.05 0.17
CV (%) 5.77 4.92 3.28 6.00 4.39 4.82
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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