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產(chǎn)品名稱：Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) Sandwich ELISA (STJE0019741)
產(chǎn)品型號：STJE0019741
產(chǎn)品廠商：ST john's laboratory
產(chǎn)品文檔：

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簡單介紹：

Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) Sandwich ELISA (STJE0019741)

詳情介紹：

Applications:	ELISA
Reactivity:	Mouse
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25-2000pg/mL
Short Description:	This RANTES Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Ccl5
Gene ID:	20304
Uniprot ID:	CCL5_MOUSE
Specificity:	This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Protein Name	C-C Motif Chemokine 5 Murantes Sis-Delta Small-Inducible Cytokine A5 T-Cell-Specific Protein Rantes
Database Links	Reactome: R-MMU-380108 Reactome: -MMU-418594
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 5 ELISA kit Murantes ELISA kit Sis-Delta ELISA kit Small-Inducible Cytokine A5 ELISA kit T-Cell-Specific Protein Rantes ELISA kit Ccl5 ELISA kit Scya5 ELISA kit
Specificity	This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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