產(chǎn)品詳情
簡單介紹：
Mouse Postn (Periostin) Sandwich ELISA (STJE0004672)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This Postn Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Postn |
| Gene ID: | 50706 |
| Uniprot ID: | POSTN_MOUSE |
| Specificity: | This kit recognizes Mouse POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Mouse POSTN/OSF-2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report in human, does not contain vitamin K-dependent gamma-carboxyglutamate residues. |
| Function | Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. |
| Protein Name | Periostin Pn Osteoblast-Specific Factor 2 Osf-2 |
| Cellular Localisation | Golgi Apparatus Secreted Extracellular Space Extracellular Matrix Colocalizes With Bmp1 In The Golgi |
| Alternative ELISA Names | Periostin ELISA kit Pn ELISA kit Osteoblast-Specific Factor 2 ELISA kit Osf-2 ELISA kit Postn ELISA kit Osf2 ELISA kit |
| Specificity | This kit recognizes Mouse POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Mouse POSTN/OSF-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 87-101 | 94 |
| Cell culture media (n=8) | 85-98 | 90 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.41 | 4.38 | 0.49 | 1.28 | 4.53 |
| Standard deviation | 0.03 | 0.08 | 0.16 | 0.03 | 0.07 | 0.22 |
| CV (%) | 6.00 | 5.67 | 3.65 | 6.12 | 5.47 | 4.86 |
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