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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse PLA2G3 (Phospholipase A2, Group III) Sandwich ELISA Kit Ready-To-Use (STJE0017917)

  • 產(chǎn)品型號(hào)：STJE0017917
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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簡單介紹：
Mouse PLA2G3 (Phospholipase A2, Group III) Sandwich ELISA Kit Ready-To-Use (STJE0017917)
詳情介紹：
Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.27ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This PLA2G3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Pla2g3
Gene ID: 237625
Uniprot ID: PA2G3_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma or other biological fluids.
Tissue Specificity Expressed in dermal mast cells (at protein level). Highly expressed in EPCAM-positive colonic epithelial cells throughout the proximal to distal colon (at protein level). Expressed at lower levels in ileum section of small intestine and immune organs including bone marrow, spleen and lymph nodes. Expressed in stomach and lung (at protein level). Expressed in testis and epididymis and to a lesser extent in the seminal vesicles and prostate. Detected in ovary, oviduct and uterus. Expressed in Sertoli and Leydig cells as well as in epididymal epithelium particularly in apical border of principal cells in the corpus (at protein level). Present in cauda epididymal fluid (at protein level). Expressed in central nervous system, with high levels in spinal cord and at a lesser extent in brain (at protein level).
Post Translational Modifications N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form is observed in several cell types. In several cell types, the N- and C-termini are cleaved off.
Function Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without apparent head group selectivity. Contributes to phospholipid remodeling of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells. May act in an autocrine and paracrine manner. Secreted by immature mast cells, acts on nearby fibroblasts upstream to PTDGS to synthesize prostaglandin D2 (PGD2), which in turn promotes mast cell maturation and degranulation via PTGDR. Secreted by epididymal epithelium, acts on immature sperm cells within the duct, modulating the degree of unsaturation of the fatty acyl components of phosphatidylcholines required for acrosome assembly and sperm cell motility. Facilitates the replacement of fatty acyl chains in phosphatidylcholines in sperm membranes from omega-6 and omega-9 to omega-3 polyunsaturated fatty acids (PUFAs). Coupled to lipoxygenase pathway, may process omega-6 PUFAs to generate oxygenated lipid mediators in the male reproductive tract. At pericentrosomal preciliary compartment, negatively regulates ciliogenesis likely by regulating endocytotic recycling of ciliary membrane protein. Coupled to cyclooxygenase pathway provides arachidonate to generate prostaglandin E2 (PGE2), a potent immunomodulatory lipid in inflammation and tumorigenesis. At colonic epithelial barrier, preferentially hydrolyzes phospholipids having arachidonate and docosahexaenoate at sn-2 position, contributing to the generation of oxygenated metabolites involved in colonic stem cell homeostasis. Releases C16:0 and C18:0 lysophosphatidylcholine subclasses from neuron plasma membranes and promotes neurite outgrowth and neuron survival.
Protein Name Group 3 Secretory Phospholipase A2
Group Iii Secretory Phospholipase A2
Giii Spla2
Spla2-Iii
Phosphatidylcholine 2-Acylhydrolase 3
Database Links Reactome: R-MMU-1482788
Reactome: -MMU-1482839
Reactome: -MMU-1482925
Cellular Localisation Secreted
Cell Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Recycling Endosome
Localized At Pericentrosomal Preciliary Compartment
Alternative ELISA Names Group 3 Secretory Phospholipase A2 ELISA kit
Group Iii Secretory Phospholipase A2 ELISA kit
Giii Spla2 ELISA kit
Spla2-Iii ELISA kit
Phosphatidylcholine 2-Acylhydrolase 3 ELISA kit
Pla2g3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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