產(chǎn)品詳情
簡(jiǎn)單介紹：
Mouse PCX (Podocalyxin) CLIA Kit (STJC0001405)
詳情介紹：
| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This mouse PCX kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Podxl |
| Gene ID: | 27205 |
| Uniprot ID: | PODXL_MOUSE |
| Specificity: | This kit recognizes Mouse PCX in samples. No significant cross-reactivity or interference between Mouse PCX and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in liver cells and hematopoietic cells (at protein level). Glomerular epithelium cell (podocyte). |
| Post Translational Modifications | N- and O-linked glycosylated. Sialoglycoprotein. |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Protein Name | Podocalyxin Podocalyxin-Like Protein 1 Pc Pclp-1 |
| Cellular Localisation | Apical Cell Membrane Cell Projection Microvillus Membrane Raft Lamellipodium Filopodium Ruffle Membrane Single-Pass Type I Membrane Protein In Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet Polarized Colocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial Polarization Colocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma Membrane Its Association With The Membrane Raft Is Transient Forms Granular Punctuated Pattern Forming Patches Preferentially Adopting A Polar Distribution Located On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial Cells Colocalizes With The Submembrane Actin Of Lamellipodia Particularly Associated With Ruffles Colocalizes With Vinculin At Protrusions Of Cells Colocalizes With Itgb1 Colocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium Cells Colocalizes With Actin Filaments Ezr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli Form Colocalizes With Pard3 Prkci Exoc5 Ocln Rab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis |
| Alternative CLIA Names | Podocalyxin CLIA kit Podocalyxin-Like Protein 1 CLIA kit Pc CLIA kit Pclp-1 CLIA kit Podxl CLIA kit Pclp1 CLIA kit |
| Specificity | This kit recognizes Mouse PCX in samples. No significant cross-reactivity or interference between Mouse PCX and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 88-102 | 94 |
| EDTA plasma(n=8) | 92-104 | 98 |
| Cell culture media(n=8) | 92-107 | 99 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.09 | 189.80 | 996.90 | 107.37 | 189.40 | 1027.29 |
| Standard deviation | 10.62 | 21.58 | 68.89 | 12.17 | 15.64 | 74.58 |
| CV (%) | 10.11 | 11.37 | 6.91 | 11.33 | 8.26 | 7.26 |
相關(guān)信息
![Anti-14-3-3 beta/Theta antibody (1-100) [S1MR] (STJ11103651)](http://y1.yzimgs.com/uploads/465546/2024520-20551366.png?imageView2/2/w/200/h/200)
- 細(xì)胞培養(yǎng)玄學(xué)-MRC-5細(xì)胞 2025-01-07
- Ocean NanoTech定制的詳細(xì)步驟 2021-10-31
- click chemistry tools數(shù)據(jù)結(jié)果比... 2021-10-31
- LaysanBio代理商相關(guān)文獻(xiàn)資料2020 2021-10-31
- Abbiotec代理的產(chǎn)品得到重復(fù)驗(yàn)證2020 2021-10-31
- zyagen Laboratories代理的產(chǎn)品得到... 2021-06-20
- Toxin Technologies代理商相關(guān)文獻(xiàn)資... 2021-03-14
- 熱烈祝賀玉博生物正式代理biocolor公司試劑產(chǎn)品... 2021-03-14
- 熱烈祝賀玉博生物正式代理cytognos公司試劑產(chǎn)品... 2021-03-14
- Gelest代理的產(chǎn)品得到重復(fù)驗(yàn)證2020 2021-03-14
