產(chǎn)品詳情
簡單介紹：
Mouse P1CP (Procollagen 1 C-Terminal Propeptide) Sandwich ELISA (STJE0004360)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This P1CP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Col1a1 |
| Gene ID: | 12842 |
| Uniprot ID: | CO1A1_MOUSE |
| Specificity: | This kit recognizes Mouse P 1 CP in samples. No significant cross-reactivity or interference between Mouse P 1 CP and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide. |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Protein Name | Collagen Alpha-1(I Chain Alpha-1 Type I Collagen |
| Database Links | Reactome: R-MMU-114604 Reactome: -MMU-1442490 Reactome: -MMU-1474244 Reactome: -MMU-1650814 Reactome: -MMU-198933 Reactome: -MMU-2022090 Reactome: -MMU-202733 Reactome: -MMU-216083 Reactome: -MMU-2243919 Reactome: -MMU-3000171 Reactome: -MMU-3000178 Reactome: -MMU-430116 Reactome: -MMU-75892 Reactome: -MMU-76009 Reactome: -MMU-8874081 Reactome: -MMU-8948216 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative ELISA Names | Collagen Alpha-1(I Chain ELISA kit Alpha-1 Type I Collagen ELISA kit Col1a1 ELISA kit Cola1 ELISA kit |
| Specificity | This kit recognizes Mouse PⅠCP in samples. No significant cross-reactivity or interference between Mouse PⅠCP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 87-100 | 94 |
| EDTA plasma (n=8) | 94-112 | 102 |
| Cell culture media (n=8) | 93-107 | 100 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.26 | 321.15 | 863.49 | 94.71 | 313.21 | 836.71 |
| Standard deviation | 6.69 | 13.87 | 41.36 | 6.00 | 15.72 | 38.40 |
| CV (%) | 6.48 | 4.32 | 4.79 | 6.34 | 5.02 | 4.59 |
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