產(chǎn)品詳情
簡(jiǎn)單介紹：
Mouse OPN (Osteopontin) CLIA Kit (STJC0001278)
詳情介紹：
| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This mouse OPN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Spp1 |
| Gene ID: | 20750 |
| Uniprot ID: | OSTP_MOUSE |
| Specificity: | This kit recognizes Mouse OPN in samples. No significant cross-reactivity or interference between Mouse OPN and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin 2ar Bone Sialoprotein 1 Calcium Oxalate Crystal Growth Inhibitor Protein Early T-Lymphocyte Activation 1 Protein Minopontin Secreted Phosphoprotein 1 Spp-1 |
| Database Links | Reactome: R-MMU-1474228 Reactome: -MMU-186797 Reactome: -MMU-216083 Reactome: -MMU-381426 Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Osteopontin CLIA kit 2ar CLIA kit Bone Sialoprotein 1 CLIA kit Calcium Oxalate Crystal Growth Inhibitor Protein CLIA kit Early T-Lymphocyte Activation 1 Protein CLIA kit Minopontin CLIA kit Secreted Phosphoprotein 1 CLIA kit Spp-1 CLIA kit Spp1 CLIA kit Eta-1 CLIA kit Op CLIA kit Spp-1 CLIA kit |
| Specificity | This kit recognizes Mouse OPN in samples. No significant cross-reactivity or interference between Mouse OPN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 85-100 | 92 |
| EDTA plasma(n=8) | 92-105 | 100 |
| Cell culture media(n=8) | 88-103 | 95 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 54.55 | 123.38 | 472.15 | 54.73 | 112.60 | 445.35 |
| Standard deviation | 5.13 | 12.81 | 45.00 | 6.21 | 9.18 | 48.10 |
| CV (%) | 9.40 | 10.38 | 9.53 | 11.35 | 8.15 | 10.80 |
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