產(chǎn)品詳情
簡單介紹：
Mouse OPG (Osteoprotegerin) Sandwich ELISA (STJE0019752)
詳情介紹：
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 62.87pg/mL |
| Detection Limit: | 125-8000pg/mL |
| Short Description: | This OPG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Tnfrsf11b |
| Gene ID: | 18383 |
| Uniprot ID: | TR11B_MOUSE |
| Specificity: | This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 11b Osteoclastogenesis Inhibitory Factor Osteoprotegerin |
| Database Links | Reactome: R-MMU-5669034 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Tumor Necrosis Factor Receptor Superfamily Member 11b ELISA kit Osteoclastogenesis Inhibitory Factor ELISA kit Osteoprotegerin ELISA kit Tnfrsf11b ELISA kit Ocif ELISA kit Opg ELISA kit |
| Specificity | This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 90-102 | 96 |
| EDTA plasma (n=8) | 88-100 | 94 |
| Cell culture media (n=8) | 87-102 | 94 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 437.57 | 693.40 | 3412.09 | 452.19 | 753.49 | 3342.64 |
| Standard deviation | 29.80 | 37.10 | 172.31 | 29.48 | 33.30 | 105.29 |
| CV (%) | 6.81 | 5.35 | 5.05 | 6.52 | 4.42 | 3.15 |
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