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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse NGB (Neuroglobin) CLIA Kit (STJC0001374)

  • 產(chǎn)品型號：STJC0001374
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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簡單介紹：
Mouse NGB (Neuroglobin) CLIA Kit (STJC0001374)
詳情介紹：
Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This mouse NGB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Ngb
Gene ID: 64242
Uniprot ID: NGB_MOUSE
Specificity: This kit recognizes Mouse NGB in samples. No significant cross-reactivity or interference between Mouse NGB and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Predominantly expressed in brain.
Post Translational Modifications A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO. Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.
Function Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
Protein Name Neuroglobin
Database Links Reactome: R-MMU-8981607
Cellular Localisation Perikaryon
Cytoplasm
Mitochondrion
Alternative CLIA Names Neuroglobin CLIA kit
Ngb CLIA kit
Specificity This kit recognizes Mouse NGB in samples. No significant cross-reactivity or interference between Mouse NGB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma(n=8) 86-101 93
Cell culture media(n=8) 85-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.04 2.22 7.74 1.09 2.14 7.96
Standard deviation 0.10 0.18 0.82 0.11 0.18 0.64
CV (%) 9.62 8.11 10.59 10.09 8.41 8.04
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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