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產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouse Muc1 (Mucin-1) Sandwich ELISA (STJE0005230)

  • 產(chǎn)品型號：STJE0005230
  • 產(chǎn)品廠商：ST john's laboratory
  • 產(chǎn)品文檔：
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簡單介紹：
Mouse Muc1 (Mucin-1) Sandwich ELISA (STJE0005230)
詳情介紹：
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Muc1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Muc1
Gene ID: 17829
Uniprot ID: MUC1_MOUSE
Specificity: This kit recognizes Mouse MUC1 in samples. No significant cross-reactivity or interference between Mouse MUC1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Probably both N- and extensively O-glycosylated (in repeat region). Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Ectodomain shedding is mediated by ADAM17 in uterine epithelial cells. Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane. Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Csk- or Src- or EGFR-mediated phosphorylation on Tyr-604 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-602 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, Src and NF-kappaB pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Regulates P53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of P53 and represses P53 activity.
Protein Name Mucin-1
Muc-1
Episialin
Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha
Muc1-Nt
Muc1-Alpha - Mucin-1 Subunit Beta
Muc1-Beta
Muc1-Ct
Database Links Reactome: R-MMU-913709
Reactome: -MMU-977068
Cellular Localisation Apical Cell Membrane
Single-Pass Type I Membrane Protein
Exclusively Located In The Apical Domain Of The Plasma Membrane Of Highly Polarized Epithelial Cells
After Endocytosis
Internalized And Recycled To The Cell Membrane
Located To Microvilli And To The Tips Of Long Filopodial Protusions
Mucin-1 Subunit Beta: Cell Membrane
Cytoplasm
Nucleus
On Egf And Pdgfrb Stimulation
Transported To The Nucleus Through Interaction With Ctnnb1
A Process Which Is Stimulated By Phosphorylation
On Hrg Stimulation
Colocalizes With Jup/Gamma-Catenin At The Nucleus
Some Transportation To The Nucleus When Infected With C
Jejeuni
Alternative ELISA Names Mucin-1 ELISA kit
Muc-1 ELISA kit
Episialin ELISA kit
Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha ELISA kit
Muc1-Nt ELISA kit
Muc1-Alpha - Mucin-1 Subunit Beta ELISA kit
Muc1-Beta ELISA kit
Muc1-Ct ELISA kit
Muc1 ELISA kit
Muc-1 ELISA kit
Specificity This kit recognizes Mouse MUC1 in samples. No significant cross-reactivity or interference between Mouse MUC1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma (n=8) 90-100 95
Cell culture media (n=8) 84-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.49 0.89 4.96 0.53 0.87 4.99
Standard deviation 0.03 0.04 0.22 0.03 0.05 0.25
CV (%) 6.12 4.49 4.44 5.66 5.75 5.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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