產(chǎn)品詳情
簡(jiǎn)單介紹：
Mouse MIF (Macrophage Migration Inhibitory Factor) CLIA Kit (STJC0001359)
詳情介紹：
| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This mouse MIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Mif |
| Gene ID: | 17319 |
| Uniprot ID: | MIF_MOUSE |
| Specificity: | This kit recognizes Mouse MIF in samples. No significant cross-reactivity or interference between Mouse MIF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
| Protein Name | Macrophage Migration Inhibitory Factor Mif Delayed Early Response Protein 6 Der6 Glycosylation-Inhibiting Factor Gif L-Dopachrome Isomerase L-Dopachrome Tautomerase Phenylpyruvate Tautomerase |
| Database Links | Reactome: R-MMU-202733 Reactome: -MMU-6798695 |
| Cellular Localisation | Secreted Cytoplasm Does Not Have A Cleavable Signal Sequence And Is Secreted Via A Specialized Non-Classical Pathway Secreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps) Or By M Tuberculosis Antigens |
| Alternative CLIA Names | Macrophage Migration Inhibitory Factor CLIA kit Mif CLIA kit Delayed Early Response Protein 6 CLIA kit Der6 CLIA kit Glycosylation-Inhibiting Factor CLIA kit Gif CLIA kit L-Dopachrome Isomerase CLIA kit L-Dopachrome Tautomerase CLIA kit Phenylpyruvate Tautomerase CLIA kit Mif CLIA kit |
| Specificity | This kit recognizes Mouse MIF in samples. No significant cross-reactivity or interference between Mouse MIF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 98-112 | 104 |
| EDTA plasma(n=8) | 95-108 | 101 |
| Cell culture media(n=8) | 85-100 | 91 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 25.18 | 50.80 | 183.34 | 22.79 | 47.95 | 176.46 |
| Standard deviation | 3.19 | 4.64 | 12.34 | 1.93 | 4.33 | 20.20 |
| CV (%) | 12.67 | 9.13 | 6.73 | 8.47 | 9.03 | 11.45 |
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